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Pro EZLC Method Translator

Column

Original

Translation

Length
mm
Inner Diameter
mm
Particle Size
µm

Volume Effects

Injection Volume
µL
Dwell Volume
mL
Extra-Column Volume
µL

Method Program

Isocratic
Gradient
Steps (2-8)
Time
(min)
%BFlow
(mL/min)
Time
(min)
%BFlow
(mL/min)

Results

Speed Gain
x
Back Pressure
x

Critical Pair Resolution
Rs
Compound Retention Time
min

Injections
Total Time
min
Solvent Usage
mL

Interested in decreasing LC run times or solvent consumption? The Pro EZLC Method Translator lets you explore the benefits a change in column dimension and/or particle size can offer. It also predicts new retention times and calculates critical pair resolutions to determine if a new column and method will deliver the performance you need

Using the Volume Effects Fields

Providing an original Injection Volume ensures a properly scaled value for the new column dimensions. Dwell Volume affects Retention Time calculations. Extra-Column Volume affects Critical Pair Resolution calculations. Both are instrument-specific values found in the instrument’s documentation or by contacting the vendor (see Glossary for more details). Providing instrument-specific values will result in the most accurate retention time and resolution calculations.

Note: Original and translated methods must use columns with the same stationary phase, mobile phase, and particle type (i.e. SPP or FPP).

DOWNLOAD
ProEZLC Method Translator
For Windows 10/8/7/Vista/XP